Detection of glycosylation modification levels based on polymerase chain reaction

TAN Zengqi; ZHANG Huina; CAO Lin

doi:10.16152/j.cnki.xdxbzr.2025-06-014

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Detection of glycosylation modification levels based on polymerase chain reaction

更新时间：2026-01-02

- Detection of glycosylation modification levels based on polymerase chain reaction
- Journal of Northwest University (Natural Science Edition) Vol. 55, Issue 6, Pages: 1364-1374(2025)
- 作者机构：
  
  1.西北大学　医学院/陕西省生物技术与生化工程重点实验室，陕西　西安　710069
  2.西北大学　生命科学学院/西部资源生物与现代生物技术教育部重点实验室，陕西　西安　710069
- 作者简介：
- 基金信息：
- DOI：10.16152/j.cnki.xdxbzr.2025-06-014
  CLC： Q53
- Received：01 September 2025，
  
  Published：25 December 2025
- 稿件说明：
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TAN Zengqi, ZHANG Huina, CAO Lin. Detection of glycosylation modification levels based on polymerase chain reaction[J]. Journal of Northwest University (Natural Science Edition), 2025, 55(6): 1364-1374.
DOI：

TAN Zengqi, ZHANG Huina, CAO Lin. Detection of glycosylation modification levels based on polymerase chain reaction[J]. Journal of Northwest University (Natural Science Edition), 2025, 55(6): 1364-1374. DOI： 10.16152/j.cnki.xdxbzr.2025-06-014.

摘要

糖基化是一种非常重要的蛋白质翻译后修饰，人体内超过50%的蛋白具有糖基化修饰，能够影响蛋白质的生物学功能参与多种生物学过程。在癌症、炎症或代谢性疾病的发生与进展等多种生理病理过程中会发生糖基化修饰的异常，因此，糖基化修饰的检测至关重要。经典的糖基化修饰检测方法，如流式细胞术、质谱等方法对于痕量的糖基化信号难以检测。该文开发了一种基于DNA扩增的糖链检测方法，将识别糖基化修饰的凝集素偶联单链DNA（ssDNA），凝集素与糖链的识别与结合把“糖信号”转化为“核酸信号”，以ssDNA为模板进行PCR级联放大，能够实现痕量的糖基化信号的检测，为糖生物学研究和临床糖组学标志物发现提供了新的视角。

Abstract

Glycosylation is an essential post-translational modification. Over 50% of proteins in the human body undergo glycosylation

which can influence their biological functions and participate in various biological processes. Abnormal glycosylation occurs in a range of physiological and pathological processes

such as cancer

inflammation

or metabolic diseases

making the detection of glycosylation essential. Classical methods for detecting glycosylation

like flow cytometry and mass spectrometry

often struggle with trace-level glycosylation signals. This study reports a DNA amplification-based method for glycan detection. Lectins that recognize and bind certain glycosylations are labeled with single-stranded DNA (ssDNA). The specific binding between lectins and glycans convert the "glycan signal" into a "nucleic acid signal". Using the ssDNA as a template for PCR amplification enables the detection of trace glycosylation signals

offering a new perspective for glycobiology research and the discovery of clinical glycomarker.

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references

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