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Trimethoprim resistance in
Pseudomonas aeruginosa is instigated by genetic mutations in theprtN gene- Journal of Northwest University (Natural Science Edition) Vol. 54, Issue 5, Pages: 909-918(2024)
- 作者机构：
西北大学 生命科学学院/西部资源生物与现代生物技术教育部重点实验室，陕西 西安 710069
- 作者简介：
- 基金信息：
DOI：10.16152/j.cnki.xdxbzr.2024-05-013
CLC： R378Published：25 October 2024，
Received：10 August 2024，
- 稿件说明：
移动端阅览
XIAO Yue, WANG Chong, SI Yujie, et al. Trimethoprim resistance in
Pseudomonas aeruginosa is instigated by genetic mutations in theprtN gene. [J]. Journal of Northwest University (Natural Science Edition), 2024,54(5):909-918.XIAO Yue, WANG Chong, SI Yujie, et al. Trimethoprim resistance in
Pseudomonas aeruginosa is instigated by genetic mutations in theprtN gene. [J]. Journal of Northwest University (Natural Science Edition), 2024,54(5):909-918. DOI： 10.16152/j.cnki.xdxbzr.2024-05-013.
摘要
耐药铜绿假单胞菌（PAO1）在临床感染过程中造成非常棘手的问题，研究其耐药机制有助于临床的治疗。研究结果表明，相对于野生型，Δ
prtN
表现出明显的甲氧苄啶（Tmp）抗性。为了了解其抗性出现的机理，测定了Tmp作用靶点
folA
的表达情况。相比于PAO1
folA
在Δ
prtN
中的表达并未升高，反而有所下降。进一步研究发现，PrtN调控的S型绿脓杆菌素基因和
prtN
的双突变体对Tmp的抗性稍有降低，而脂多糖缺陷菌株Δ
wbpL
对Tmp的抗性略有升高。在Δ
prtN
中活性氧（ROS）相关基因（
oxyR
，
katA
，
ahpC
）的表达水平、生物被膜及外排泵相关抗生素抗性检测结果都显示与野生型无显著性差异。综上，
prtN
基因突变引起的Tmp抗性可能是通过PrtN调控S型绿脓杆菌素及脂多糖相关靶标作用的结果。
Abstract
Antibiotic-resistant
Pseudomonas aeruginosa
(PAO1) poses a significant challenge in clinical infections
highlighting the importance of studying its resistance mechanisms for improving clinical treatments. Our study found that the Δ
prtN
exhibited significant resistance to tr
imethoprim (Tmp) compared to the wild type. To elucidate the underlying mechanism of this resistance
we assessed the expression of
folA
the target of Tmp. Interestingly
folA
expression in Δ
prtN
was not elevated
instead but rather decreased compared to the PAO1 strain. Further investigations revealed that a double mutant lacking both the PrtN-regulated S type pyocin biosynthesis gene and
prtN
exhibited slightly reduced Tmp resistance. In contrast
the lipopolysaccharide-deficient strain Δ
wbpL
showed slightly increased Tmp resistance. In Δ
prtN
the expression levels of reactive oxygen species(ROS)-related genes (
oxyR
katA
ahpC
)
biofilm formation
and antibiotic resistance associated with efflux pumps showed no significant difference compared to the wild type. In conclusion
the Tmp resistance observed in the
prtN
mutant is likely due to the regulatory effects of PrtN on S-type pyocins and lipopolysaccharide-related targets.
关键词
绿脓杆菌素prtN甲氧苄啶脂多糖ROS
Keywords
pyocinprtNtrimethoprimLPSROS
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