基于聚合酶链式反应检测糖基化修饰水平

谭增琦; 张慧娜; 曹琳

doi:10.16152/j.cnki.xdxbzr.2025-06-014

您当前的位置：

首页 >

文章列表页 >

基于聚合酶链式反应检测糖基化修饰水平

青年编委专刊 | 更新时间：2026-01-02

- 基于聚合酶链式反应检测糖基化修饰水平
- Detection of glycosylation modification levels based on polymerase chain reaction
- 西北大学学报（自然科学版） 2025年55卷第6期页码：1364-1374
- 作者机构：
  
  1.西北大学　医学院/陕西省生物技术与生化工程重点实验室，陕西　西安　710069
  2.西北大学　生命科学学院/西部资源生物与现代生物技术教育部重点实验室，陕西　西安　710069
- 作者简介：
  
  [ "谭增琦，西北大学医学院副教授，博士生导师。现任美国细胞外囊泡协会会刊Extracellular Vesicle青年编委，陕西省抗癌协会肿瘤筛查及早诊早治专委会委员及西安医学会医学教育分会委员。从事肿瘤糖生物学方向研究，围绕糖基化修饰在肿瘤微环境中的生物学功能，在Journal of Extracellular Vesicles、Journal of Experimental Clinical Cancer Research、Journal of Pineal Research、Journal of Proteome Research等期刊上发表多篇研究论文。主持多项国家自然科学基金及省部级科研项目。" ]
- 基金信息：
  
  国家自然科学基金(32271338)
- DOI：10.16152/j.cnki.xdxbzr.2025-06-014
  中图分类号： Q53
- 收稿：2025-09-01，
  
  纸质出版：2025-12-25
- 稿件说明：
移动端阅览
谭增琦, 张慧娜, 曹琳. 基于聚合酶链式反应检测糖基化修饰水平[J]. 西北大学学报（自然科学版）, 2025,55(6):1364-1374.

TAN Zengqi, ZHANG Huina, CAO Lin. Detection of glycosylation modification levels based on polymerase chain reaction[J]. Journal of Northwest University (Natural Science Edition), 2025, 55(6): 1364-1374.
谭增琦, 张慧娜, 曹琳. 基于聚合酶链式反应检测糖基化修饰水平[J]. 西北大学学报（自然科学版）, 2025,55(6):1364-1374. DOI： 10.16152/j.cnki.xdxbzr.2025-06-014.

TAN Zengqi, ZHANG Huina, CAO Lin. Detection of glycosylation modification levels based on polymerase chain reaction[J]. Journal of Northwest University (Natural Science Edition), 2025, 55(6): 1364-1374. DOI： 10.16152/j.cnki.xdxbzr.2025-06-014.

摘要

糖基化是一种非常重要的蛋白质翻译后修饰，人体内超过50%的蛋白具有糖基化修饰，能够影响蛋白质的生物学功能参与多种生物学过程。在癌症、炎症或代谢性疾病的发生与进展等多种生理病理过程中会发生糖基化修饰的异常，因此，糖基化修饰的检测至关重要。经典的糖基化修饰检测方法，如流式细胞术、质谱等方法对于痕量的糖基化信号难以检测。该文开发了一种基于DNA扩增的糖链检测方法，将识别糖基化修饰的凝集素偶联单链DNA（ssDNA），凝集素与糖链的识别与结合把“糖信号”转化为“核酸信号”，以ssDNA为模板进行PCR级联放大，能够实现痕量的糖基化信号的检测，为糖生物学研究和临床糖组学标志物发现提供了新的视角。

Abstract

Glycosylation is an essential post-translational modification. Over 50% of proteins in the human body undergo glycosylation

which can influence their biological functions and participate in various biological processes. Abnormal glycosylation occurs in a range of physiological and pathological processes

such as cancer

inflammation

or metabolic diseases

making the detection of glycosylation essential. Classical methods for detecting glycosylation

like flow cytometry and mass spectrometry

often struggle with trace-level glycosylation signals. This study reports a DNA amplification-based method for glycan detection. Lectins that recognize and bind certain glycosylations are labeled with single-stranded DNA (ssDNA). The specific binding between lectins and glycans convert the "glycan signal" into a "nucleic acid signal". Using the ssDNA as a template for PCR amplification enables the detection of trace glycosylation signals

offering a new perspective for glycobiology research and the discovery of clinical glycomarker.

关键词

Keywords

references

XU X M , PENG Q , JIANG X J , et al . Altered glycosylation in cancer: Molecular functions and therapeutic potential [J ] . Cancer Communications , 2024 , 44 ( 11 ): 1316 - 1336 .

OHTSUBO K , MARTH J D . Glycosylation in cellular mechanisms of health and disease [J ] . Cell , 2006 , 126 ( 5 ): 855 - 867 .

STRUWE W B , ROBINSON C V . Relating glycoprotein structural heterogeneity to function-insights from native mass spectrometry [J ] . Current Opinion in Structural Biology , 2019 , 58 : 241 - 248 .

LIU K F , TAN S G , JIN W J , et al . N-glycosylation of PD-1 promotes binding of camrelizumab [J ] . EMBO Reports , 2020 , 21 ( 12 ): e51444 .

REN X T , WU J P , LI J , et al . Bisecting GlcNAc enhances CD8 + T cell-mediated killing of breast cancer by suppressing PD-L1 expression and its binding to PD-1 [J ] . Experimental Hematology & Oncology , 2025 , 14 ( 1 ): 102 .

KUKAN E N , FABIANO G L , COBB B A . Siglecs as modulators of macrophage phenotype and function [J ] . Seminars in Immunology , 2024 , 73 : 101887 .

YAO H Y Y , WANG J Q , YIN J Y , et al . A review of NMR analysis in polysaccharide structure and conformation: Progress, challenge and perspective [J ] . Food Research International , 2021 , 143 : 110290 .

YU W H , ZHAO X L , JALLOH A S , et al . Chemoenzymatic measurement of LacNAc in single-cell multiomics reveals it as a cell-surface indicator of glycolytic activity of CD8 + T cells [J ] . Journal of the American Chemical Society , 2023 , 145 ( 23 ): 12701 - 12716 .

HAN C C , JIN L , MEI Y D , et al . Endoplasmic reticulum stress inhibits cell cycle progression via induction of p27 in melanoma cells [J ] . Cellular Signalling , 2013 , 25 ( 1 ): 144 - 149 .

HOU H L , SUN H F , LU P , et al . Tunicamycin potentiates cisplatin anticancer efficacy through the DPAGT1/Akt/ABCG2 pathway in mouse Xenograft models of human hepatocellular carcinoma [J ] . Molecular Cancer Therapeutics , 2013 , 12 ( 12 ): 2874 - 2884 .

HSU J L , CHIANG P C , GUH J H . Tunicamycin induces resistance to camptothecin and etoposide in human hepatocellular carcinoma cells: Role of cell-cycle arrest and GRP78 [J ] . Naunyn-Schmiedeberg's Archives of Pharmacology , 2009 , 380 ( 5 ): 373 - 382 .

MATSUMIYA W , KUSUHARA S , HAYASHIBE K , et al . Forskolin modifies retinal vascular development in Mrp4-knockout mice [J ] . Investigative Ophthalmology & Visual Science , 2012 , 53 ( 13 ): 8029 - 8035 .

董芳 , 肖凡 , 魏红山 . 平分型GlcNAc糖基化修饰的生物学功能 [J ] . 医学分子生物学杂志 , 2009 , 6 ( 2 ): 138 - 143 .

DONG F , XIAO F , WEI H S . Bisecting N-acetylglucosamine and its biological functions [J ] . Journal of Medical Molecular Biology , 2009 , 6 ( 2 ): 138 - 143 .

TAN Z Q , CAO L , WU Y R , et al . Bisecting GlcNAc modification diminishes the pro-metastatic functions of small extracellular vesicles from breast cancer cells [J ] . Journal of Extracellular Vesicles , 2020 , 10 ( 1 ): e12005 .

HE S S , BHATT R , BROWN C , et al . High-plex imaging of RNA and proteins at subcellular resolution in fixed tissue by spatial molecular imaging [J ] . Nature Biotechnology , 2022 , 40 ( 12 ): 1794 - 1806 .

KEARNEY C J , VERVOORT S J , RAMSBOTTOM K M , et al . SUGAR-seq enables simultaneous detection of glycans, epitopes, and the transcriptome in single cells [J ] . Science Advances , 2021 , 7 ( 8 ): eabe3610 .

WANG Y C , NAKAGAWA M , GARITAONANDIA I , et al . Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis [J ] . Cell Research , 2011 , 21 ( 11 ): 1551 - 1563 .

SOJITRA M , SCHMIDT E N , LIMA G M , et al . Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA) [J ] . Nature Protocols , 2025 , 20 ( 4 ): 989 - 1019 .

TONG Q H , TAO T , XIE L Q , et al . ELISA-PLA: A novel hybrid platform for the rapid, highly sensitive and specific quantification of proteins and post-translational modifications [J ] . Biosensors and Bioelectronics , 2016 , 80 : 385 - 391 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据